NMR Experiments and Procedures

NOTE that these instructions are based on VnmrJ 2.2D with chempack active. 
 
If you want to activate chempack (a package of programs for NMR experiments and macros that help set them up), draw down the "edit" menu, release on "applications". Look in the wide middle column named "Application Directory", look for "/vnmr/chempack". Check in the same row to the left of the chempack entry to see if it is "enabled". If not, use the arrowhead there to select 'enabled'. Click on "OK" at the bottom of the dialog box. Exit VnmrJ and relaunch it. This procedure is fully reversible, you can alsways disable chempack if you do not like it. 
 
Prof. Miller is happy to provide guidance with any of the following, however her response time is often limited by additional commitments. Dr. Justin Mobley can be of assistance with running the following experiments. However, please notify Dr. Mobley in advance (i.e. please email Dr. Mobley 1 week prior to performing the experiments).
 

Collecting a basic 1H 1d

Brief Instructions

Detailed Instructions

Optmizing a 1H 1D, including calibration of the pw90

Brief Instructions

Detailed Instructions

Measuring the T1

(characteristic time required for recovery of 1H magnetization)

Detailed Instructions

Using linear prediction and weighting functions

Detailed Instructions

Selective pulses and selective 1D-TOCSY

(connectivity through bonds between 1Hs in a spin system)

This is a great way to identify resonances belonging to the same spin system as a given resonance that is well separated from others. Use care not to employ too strong or long a spin lock. Keep the spinlock power < 50 and duration mixT < 0.1 .

Detailed Instructions

2D TOCSY

(connectivity through bonds between 1Hs in a spin system)

Use care not to employ too strong or long a spin lock. Keep the spinlock power < 50 and duration < 0.1 .

Detailed Instructions

"Wet" solvent suppression with selective pulses.

Detailed Instructions

NOESY

(connectivity between 1H through space, up to approx. 6 Angstrom apart)

Detailed Instructions

COSY

(connectivity through bonds between 1Hs up to four bonds apart)

Detailed Instructions

13C 1D spectra

Detailed Instructions

13C HSQC

(one bond correlations between 1H and 13C)

Detailed Instructions

13C HMBC

(multiple bond correlations between 1H and 13C)

Detailed Instructions

Advanced options for simultaneously displaying and comparing multiple spectra

Detailed Instructions

How to measure diffusion coefficients

Detailed Instructions

This procedure requires some assistance. Please schedule help from Prof. Miller.

Selective 1D nOe

Detailed instructions.

This procedure requires some assistance, please schedule help from Prof. Miller or Dr. Mobley.

 

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