Anne-Frances Miller

afm's picture
  • Professor of Chemistry, and of Biochemistry
  • Biological
  • Chemistry
afmill3r2@gmail.com
CP 113, Chemistry-Physics Building, Lab CP 106
email: afm@uky.edu
Research Site
Research Interests:
  • Enzymes
  • redox catalysis
  • flavin-mediated reactions
  • flavin redox
  • and photochemistry
  • enzyme-functionalized electrodes and membranes
  • spectroscopy
  • mechanistic enzymology
  • enzyme engineering
Education

Ph.D Yale University, 1989

Postdoctoral, Massachusetts Institute of Technology, 1989-90

Postdoctoral Fellow, Brandeis Univ. 1990-92

Research
  • Enzymatic Redox Catalysis - Miller Group website
  • See the Group Website !! (College website maintained as finances allow)
Graduate Training

Bioinorganic Chemistry, Spectroscopy, Biochemistry

PubMed Publications*: 
  • Mohamed-Raseek, N ;Miller, AF "Contrasting roles for two conserved arginines: Stabilizing flavin semiquinone or quaternary structure, in bifurcating electron transfer flavoproteins." The Journal of biological chemistry 298, 4 (2022): 101733. Details. Full text
  • Varner, TA ;Mohamed-Raseek, N ;Miller, AF "Assignments of <sup>19</sup>F NMR resonances and exploration of dynamics in a long-chain flavodoxin." Archives of biochemistry and biophysics 703, (2021): 108839. Details. Full text
  • Duan, HD ;Khan, SA ;Miller, AF "Photogeneration and reactivity of flavin anionic semiquinone in a bifurcating electron transfer flavoprotein." Biochimica et biophysica acta. Bioenergetics 1862, 7 (2021): 148415. Details. Full text
  • Kar, RK ;Chasen, S ;Mroginski, MA ;Miller, AF "Tuning the Quantum Chemical Properties of Flavins via Modification at C8." The journal of physical chemistry. B 125, 46 (2021): 12654-12669. Details. Full text
  • Pitsawong, W ;Chenprakhon, P ;Dhammaraj, T ;Medhanavyn, D ;Sucharitakul, J ;Tongsook, C ;van Berkel, WJH ;Chaiyen, P ;Miller, AF "Tuning of p<i>K<sub>a</sub></i> values activates substrates in flavin-dependent aromatic hydroxylases." The Journal of biological chemistry 295, 12 (2020): 3965-3981. Details. Full text
  • Duan, HD ;Mohamed-Raseek, N ;Miller, AF "Spectroscopic evidence for direct flavin-flavin contact in a bifurcating electron transfer flavoprotein." The Journal of biological chemistry 295, 36 (2020): 12618-12634. Details. Full text
  • Mohamed-Raseek, N ;Duan, HD ;Hildebrandt, P ;Mroginski, MA ;Miller, AF "Spectroscopic, thermodynamic and computational evidence of the locations of the FADs in the nitrogen fixation-associated electron transfer flavoprotein." Chemical science 10, 33 (2019): 7762-7772. Details. Full text
  • Schut, GJ ;Mohamed-Raseek, N ;Tokmina-Lukaszewska, M ;Mulder, DW ;Nguyen, DMN ;Lipscomb, GL ;Hoben, JP ;Patterson, A ;Lubner, CE ;King, PW ;Peters, JW ;Bothner, B ;Miller, AF ;Adams, MWW "The catalytic mechanism of electron-bifurcating electron transfer flavoproteins (ETFs) involves an intermediary complex with NAD&lt;sup/&gt;." The Journal of biological chemistry 294, 9 (2019): 3271-3283. Details. Full text
  • Schut, GJ ;Mohamed-Raseek, N ;Tokmina-Lukaszewska, M ;Mulder, DW ;Nguyen, DMN ;Lipscomb, GL ;Hoben, JP ;Patterson, A ;Lubner, CE ;King, PW ;Peters, JW ;Bothner, B ;Miller, AF ;Adams, MWW "The catalytic mechanism of electron-bifurcating electron transfer flavoproteins (ETFs) involves an intermediary complex with NAD<sup/>." The Journal of biological chemistry 294, 9 (2019): 3271-3283. Details. Full text
  • Duan, HD ;Lubner, CE ;Tokmina-Lukaszewska, M ;Gauss, GH ;Bothner, B ;King, PW ;Peters, JW ;Miller, AF "Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from <i>Rhodopseudomonas palustris</i>." The Journal of biological chemistry 293, 13 (2018): 4688-4701. Details. Full text
  • Miller, AF ;Park, JT ;Ferguson, KL ;Pitsawong, W ;Bommarius, AS "Informing Efforts to Develop Nitroreductase for Amine Production." Molecules (Basel, Switzerland) 23, 2 (2018): Details. Full text
  • Lubner, CE ;Jennings, DP ;Mulder, DW ;Schut, GJ ;Zadvornyy, OA ;Hoben, JP ;Tokmina-Lukaszewska, M ;Berry, L ;Nguyen, DM ;Lipscomb, GL ;Bothner, B ;Jones, AK ;Miller, AF ;King, PW ;Adams, MWW ;Peters, JW "Mechanistic insights into energy conservation by flavin-based electron bifurcation." Nature chemical biology 13, 6 (2017): 655-659. Details. Full text
  • Das, S.;Xu, W ;Lehmler, HJ ;Miller, AF ;Knutson, BL ;Rankin, SE "Inverted Micelle-in-Micelle Configuration in Cationic/Carbohydrate Surfactant Mixtures." Chemphyschem : a European journal of chemical physics and physical chemistry 18, 1 (2017): 79-86. Details. Full text
  • Costas, AM Garcia;Poudel, S ;Miller, AF ;Schut, GJ ;Ledbetter, RN ;Fixen, KR ;Seefeldt, LC ;Adams, MWW ;Harwood, CS ;Boyd, ES ;Peters, JW "Defining Electron Bifurcation in the Electron-Transferring Flavoprotein Family." Journal of bacteriology 199, 21 (2017): Details. Full text
  • Ledbetter, RN ;Costas, AM Garcia;Lubner, CE ;Mulder, DW ;Tokmina-Lukaszewska, M ;Artz, JH ;Patterson, A ;Magnuson, TS ;Jay, ZJ ;Duan, HD ;Miller, J.;Plunkett, MH ;Hoben, JP ;Barney, BM ;Carlson, RP ;Miller, AF ;Bothner, B ;King, PW ;Peters, JW ;Seefeldt, LC "The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis." Biochemistry 56, 32 (2017): 4177-4190. Details. Full text
  • Miller, AF ;Wang, T. "A Single Outer-Sphere Mutation Stabilizes apo-Mn Superoxide Dismutase by 35 °C and Disfavors Mn Binding." Biochemistry 56, 29 (2017): 3787-3799. Details. Full text
  • Hoben, JP ;Lubner, CE ;Ratzloff, MW ;Schut, GJ ;Nguyen, DMN ;Hempel, KW ;Adams, MWW ;King, PW ;Miller, AF "Equilibrium and ultrafast kinetic studies manipulating electron transfer: A short-lived flavin semiquinone is not sufficient for electron bifurcation." The Journal of biological chemistry 292, 34 (2017): 14039-14049. Details. Full text
  • Pitsawong, W ;Haynes, CA ;RL, Koder ;Rodgers, DW ;Miller, AF "Mechanism-Informed Refinement Reveals Altered Substrate-Binding Mode for Catalytically Competent Nitroreductase." Structure (London, England : 1993) 25, 7 (2017): 978-987.e4. Details. Full text
  • Sarma, R ;Islam, MS ;Miller, AF ;Bhattacharyya, D "Layer-by-Layer-Assembled Laccase Enzyme on Stimuli-Responsive Membranes for Chloro-Organics Degradation." ACS applied materials & interfaces 9, 17 (2017): 14858-14867. Details. Full text
  • Sarma, R ;Sloan, MJ ;Miller, AF "Flavin-sensitized electrode system for oxygen evolution using photo-electrocatalysis." Chemical communications (Cambridge, England) 52, 57 (2016): 8834-7. Details. Full text
  • Peters, JW ;Miller, AF ;Jones, AK ;King, PW ;Adams, MW "Electron bifurcation." Current opinion in chemical biology 31, (2016): 146-52. Details. Full text
  • Pitsawong, W ;Hoben, JP ;Miller, AF "Understanding the broad substrate repertoire of nitroreductase based on its kinetic mechanism." The Journal of biological chemistry 289, 22 (2014): 15203-14. Details. Full text
  • Rathnayake, S ;Unrine, JM ;Judy, J ;Miller, AF ;Rao, W ;Bertsch, PM "Multitechnique investigation of the pH dependence of phosphate induced transformations of ZnO nanoparticles." Environmental science & technology 48, 9 (2014): 4757-64. Details. Full text
  • Pan, J.;Bhardwaj, M ;Nagabhyru, P.;Charlton, ND ;Higashi, RM ;Miller, AF ;Young, CA ;Grossman, RB ;Schardl, CL "Ether bridge formation in loline alkaloid biosynthesis." Phytochemistry 98, (2014): 60-8. Details. Full text
  • Miller, AF "Superoxide dismutases: ancient enzymes and new insights." FEBS letters 586, 5 (2012): 585-95. Details. Full text
  • Purushothaman, B ;Bruzek, M ;, ;Miller, AF ;Anthony, JE "Synthesis and structural characterization of crystalline nonacenes." Angewandte Chemie (International ed. in English) 50, 31 (2011): 7013-7. Details. Full text
  • Cui, D ;Koder, RL ;Dutton, PL ;Miller, AF "15N solid-state NMR as a probe of flavin H-bonding." The journal of physical chemistry. B 115, 24 (2011): 7788-98. Details. Full text
  • Miller, AF ;Yikilmaz, E ;Vathyam, S "15N-NMR characterization of His residues in and around the active site of FeSOD." Biochimica et biophysica acta 1804, 2 (2010): 275-84. Details. Full text
  • Johnston, JW ;Shamsulddin, H ;Miller, AF ;Apicella, MA "Sialic acid transport and catabolism are cooperatively regulated by SiaR and CRP in nontypeable Haemophilus influenzae." BMC microbiology 10, (2010): 240. Details. Full text
  • Miller, AF "The shortest wire." Proceedings of the National Academy of Sciences of the United States of America 105, 21 (2008): 7341-2. Details. Full text
  • Boatright, WL ;Jahan, MS ;Walters, BM ;Miller, AF ;Cui, D ;Hustedt, EJ ;Lei, Q "Carbon-centered radicals in isolated soy proteins." Journal of food science 73, 3 (2008): C222-6. Details. Full text
  • Miller, AF "Redox tuning over almost 1 V in a structurally conserved active site: lessons from Fe-containing superoxide dismutase." Accounts of chemical research 41, 4 (2008): 501-10. Details. Full text
  • Yikilmaz, E ;Porta, J ;Grove, LE ;Vahedi-Faridi, A ;Bronshteyn, Y ;Brunold, TC ;Borgstahl, GE ;Miller, AF "How can a single second sphere amino acid substitution cause reduction midpoint potential changes of hundreds of millivolts?" Journal of the American Chemical Society 129, 32 (2007): 9927-40. Details. Full text
  • RL, Koder ;Walsh, JD ;Pometun, MS ;Dutton, PL ;Wittebort, RJ ;Miller, AF "15N solid-state NMR provides a sensitive probe of oxidized flavin reactive sites." Journal of the American Chemical Society 128, 47 (2006): 15200-8. Details. Full text
  • Chellgren, BW ;Miller, AF ;Creamer, TP "Evidence for polyproline II helical structure in short polyglutamine tracts." Journal of molecular biology 361, 2 (2006): 362-71. Details. Full text
  • Yikilmaz, E ;Rodgers, DW ;Miller, AF "The crucial importance of chemistry in the structure-function link: manipulating hydrogen bonding in iron-containing superoxide dismutase." Biochemistry 45, 4 (2006): 1151-61. Details. Full text
  • Miller, AF "Superoxide dismutases: active sites that save, but a protein that kills." Current opinion in chemical biology 8, 2 (2004): 162-8. Details. Full text
  • Miller, AF ;Padmakumar, K ;Sorkin, DL ;Karapetian, A ;Vance, CK "Proton-coupled electron transfer in Fe-superoxide dismutase and Mn-superoxide dismutase." Journal of inorganic biochemistry 93, 1-2 (2003): 71-83. Details. Full text
  • Maliekal, J ;Karapetian, A ;Vance, C ;Yikilmaz, E ;Wu, Q ;Jackson, T ;Brunold, TC ;Spiro, TG ;Miller, AF "Comparison and contrasts between the active site PKs of Mn-superoxide dismutase and those of Fe-superoxide dismutase." Journal of the American Chemical Society 124, 50 (2002): 15064-75. Details. Full text
  • Koder, RL ;Haynes, CA ;Rodgers, ME ;Rodgers, DW ;Miller, AF "Flavin thermodynamics explain the oxygen insensitivity of enteric nitroreductases." Biochemistry 41, 48 (2002): 14197-205. Details. Full text
  • Yikilmaz, E ;Xie, J.;Brunold, TC ;Miller, AF "Hydrogen-bond-mediated tuning of the redox potential of the non-heme Fe site of superoxide dismutase." Journal of the American Chemical Society 124, 14 (2002): 3482-3. Details. Full text
  • Haynes, CA ;Koder, RL ;Miller, AF ;Rodgers, DW "Structures of nitroreductase in three states: effects of inhibitor binding and reduction." The Journal of biological chemistry 277, 13 (2002): 11513-20. Details. Full text
  • Koder, RL ;Oyedele, O ;Miller, AF "Retro-nitroreductase, a putative evolutionary precursor to Enterobacter cloacae strain 96-3 nitroreductase." Antioxidants & redox signaling 3, 5 (2001): 747-55. Details. Full text
  • Kelly, MA ;Chellgren, BW ;Rucker, AL ;Troutman, JM ;Fried, MG ;Miller, AF ;Creamer, TP "Host-guest study of left-handed polyproline II helix formation." Biochemistry 40, 48 (2001): 14376-83. Details. Full text
  • Schwartz, AL ;Yikilmaz, E ;Vance, CK ;Vathyam, S ;Koder, RL ;Miller, AF "Mutational and spectroscopic studies of the significance of the active site glutamine to metal ion specificity in superoxide dismutase." Journal of inorganic biochemistry 80, 3-4 (2000): 247-56. Details. Full text
  • Miller, AF ;Duan, HD ;Varner, TA ;Raseek, Mohamed "Reduction midpoint potentials of bifurcating electron transfer flavoproteins." Methods in enzymology 620, (0): 365-398. Details. Full text
  • Miller, AF "Solid-state NMR of flavins and flavoproteins." Methods in molecular biology (Clifton, N.J.) 1146, (0): 307-40. Details. Full text
  • Adejuwon, Adeneye ;Crooks, PA ;Fadhel-Albayati, Z ;Miller, AF ;Zito, SW ;Adeyemi, OO ;Agbaje, EO "Antihyperglycemic profile of erinidine isolated from Hunteria umbellata seed." African journal of traditional, complementary, and alternative medicines : AJTCAM 10, 2 (0): 189-202. Details.
* Publications are automatically pulled from pubmed.gov based on a user-specific query. Results may include incorrect citations. See: Tutorial on improving PubMed results.