Abstract: Peptides represent a rapidly expanding class of therapeutics capable of targeting protein surfaces and interfaces inaccessible to conventional small molecules. Yet our ability to study their behavior in living systems remains limited by the tools available. Traditional fluorescent labels are often too bulky to be incorporated without disrupting peptide function and target engagement. My research program develops minimally perturbative vibrational imaging tools to visualize peptide dynamics in complex biological environments. By integrating precision peptide design, bioorthogonal Raman tags and advanced spectroscopic imaging, we create platforms that enable noninvasive, chemically specific mapping of therapeutics in cells and tissues.