Bert C. Lynn

bclynn2's picture
  • Professor of Chemistry; Director, UK Mass Spectrometry Facility
  • Analytical
  • Chemistry
A053 ASTeCC Building
859-218-6529
Research Interests:
Education

Ph.D. Mississippi State University, 1987

Research

Mass spectrometry research in my group is divided into three broad areas: 1) mass spectrometry based "Omic" sciences 2) analytical methods development in chemistry and biology and 3)  instrument development.

In the “Omics” area, we are currently working in proteomics, lipidomics and metabolomics.  We continue our efforts to utilize proteomics to elucidate protein changes associated with protozoa infections, neuronal diseases such as Alzheimer’s disease and microbial solventogenesis in biofuels production.  We are also working on metabolic profiles from easily obtainable biological fluids using capillary electrophoresis mass spectrometry.

Methods development has been expanded to include biofuels and forensic problems.  We are currently developing methods to elucidate changes in lignin composition during biomass pretreatment, synthesizing model lignin oligomers and utilizing tandem mass spectrometry to identify monolignol composition and bonding.  Additionally, we are exploring the use of solid phase microextraction with GC/MS and paper spray mass spectrometry to characterize and quantify organic gunshot residue.

A variety of instrument development projects are underway including development of alternative API ionization schemes (inlet ionization) and novel CE/MS interfaces.

 


 

 

Graduate Training

Analytical Chemistry, Mass Spectrometry

Selected Publications: 

Omics and Biomarkers

  • X. Liu, M.A. Lovell and B.C. Lynn, 2006.  Detection and Quantification of Endogenous Cyclic DNA Adducts Derived from 4-hydroxynonenal in Human Brain Tissue by Isotope Dilution Capillary Liquid Chromatography Nanoelectrospray Tandem Mass Spectrometry. Chem. Res. Toxicol., 19: 710-718.
  • Fu, Y., Xiong, S., Lovell, M.A., and B.C. Lynn. 2009. Quantitative proteomic analysis of mitochondria in aging PS-1 transgenic mice. Cellular and Molecular Neurobiology 29:649-664.
  • Lynn, B.C., Wang, J., Markesbery, W.R., and M.A. Lovell. 2010. Quantitative changes in the mitochondrial proteome from subjects with mild cognitive impairment, early stage, and late stage Alzheimer's disease. Journal of Alzheimer’s Disease 19:325-339.
  • Timmons, M.D., Bradley, M.A., Lovell, M.A., and B.C. Lynn. 2011. Procedure for the isolation of mitochondria, cytosolic and nuclear material from a single piece of neurological tissue for high-throughput mass spectral analysis. Journal of Neuroscience Methods 197:279-282.
  • M.A. Bradley-Whitman, E. Abner , B.C. Lynn, M.A. Lovell. 2015. A Novel Plasma Based Biomarker of Alzheimer's Disease.  J. Alzheimers Dis., 47, 761-71.
  • M.A. Lovell, E. Abner, R. Kryscio, L. Xu, S.X. Fister, B.C. Lynn. 2015. Calcium Channel Blockers, Progression to Dementia, and Effects on Amyloid Beta Peptide Production.  Oxidative Medicine and Cellular Longevity, Article ID 787805, 9 pages.

Biomass

  • Williams, T.I., Combs, J.C., Lynn, B.C., and H.J. Strobel. 2007. Proteomic profile changes in membranes of ethanol-tolerant. Applied Microbiology Biotechnology 74:422-432.
  • Timmons, M.D., Knutson, B.L., Nokes, S.E., Strobel, H.J., and B.C. Lynn. 2009. Analysis of composition and structure of Clostridium thermocellum membranes from wild-type and ethanol-adapted strains. Applied Microbiology Biotechnology 82:929-939.
  • Li, H.F., Knutson, B.L., Nokes, S.E., Lynn, B.C., and M.D. Flythe. 2012. Metabolic control of Clostridium thermocellum via inhibition of hydrogenase activity and the glucose transport rate. Applied Microbiology Biotechnology 93(4):1777-84.
  • Harman-Ware, A.E., Crocker, M., Kaur, A.P., Meier, M.S., Kato, D., and B.C. Lynn. 2013. Pyrolysis-GC/MS of sinapyl and coniferyl alcohol. Journal of Analytical and Applied Pyrolysis 99:161-169.
  • Kato, D.M., Élia, N., Flythe, M., and B.C. Lynn. 2014. Pretreatment of lignocellulosic biomass using Fenton chemistry. Bioresource Technology 162:273-278.
PubMed Publications*: 
  • Kelley, ZD ;Lovell, MA ;Lynn, BC "Pharmacokinetic and metabolic analysis of an Alzheimer's disease therapeutic in rat serum via microfluidic CZE-MS." Biomedical chromatography : BMC 36, 1 (2022): e5243. Details. Full text
  • Dean, KR ;Novak, B ;Moradipour, M ;Tong, X ;Moldovan, D ;Knutson, BL ;Rankin, SE ;Lynn, BC "Complexation of Lignin Dimers with β-Cyclodextrin and Binding Stability Analysis by ESI-MS, Isothermal Titration Calorimetry, and Molecular Dynamics Simulations." The journal of physical chemistry. B 126, 8 (2022): 1655-1667. Details. Full text
  • Dorrani, M ;Lynn, BC "Application of lithium cationization tandem mass spectrometry for structural analysis of lignin model oligomers with β-β' and β-O-4' linkages." Analytical and bioanalytical chemistry (2022): Details. Full text
  • Asare, SO ;Dean, KR ;Lynn, BC "The study of the chromatographic behavior and a mass spectrometric approach to differentiating the diastereomer pair of the β-O-4 lignin dimer series." Analytical and bioanalytical chemistry 413, 15 (2021): 4037-4048. Details. Full text
  • Moradipour, M ;Tong, X ;Novak, B ;Kamali, P ;Asare, SO ;Lynn, BC ;Moldovan, D ;Rankin, SE ;Knutson, BL "Interaction of lignin dimers with model cell membranes: A quartz crystal microbalance and molecular dynamics simulation study." Biointerphases 16, 4 (2021): 041003. Details.
  • Belgodere, JA ;Zamin, SA ;Kalinoski, RM ;Astete, CE ;Penrod, JC ;Hamel, KM ;Lynn, BC ;Rudra, JS ;Shi, J.;Jung, JP "Correction to Modulating Mechanical Properties of Collagen-Lignin Composites." ACS applied bio materials 4, 6 (2021): 5391. Details.
  • Moradipour, M ;Chase, EK ;Khan, MA ;Asare, SO ;Lynn, BC ;Rankin, SE ;Knutson, BL "Interaction of lignin-derived dimer and eugenol-functionalized silica nanoparticles with supported lipid bilayers." Colloids and surfaces. B, Biointerfaces 191, (2020): 111028. Details. Full text
  • Tong, X ;Moradipour, M ;Novak, B ;Kamali, P ;Asare, SO ;Knutson, BL ;Rankin, SE ;Lynn, BC ;Moldovan, D "Experimental and Molecular Dynamics Simulation Study of the Effects of Lignin Dimers on the Gel-to-Fluid Phase Transition in DPPC Bilayers." The journal of physical chemistry. B 123, 39 (2019): 8247-8260. Details. Full text
  • Kelley, ZD ;Rogers, DT ;Littleton, JM ;Lynn, BC "Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures." Electrophoresis 40, 22 (2019): 2921-2928. Details.
  • Asare, SO ;Lynn, BC "A comparative study of the electrospray ionization response of β-O-4' lignin model compounds." Journal of mass spectrometry : JMS 54, 6 (2019): 540-548. Details. Full text
  • Bowman, AS ;Asare, SO ;Lynn, BC "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis for characterization of lignin oligomers using cationization techniques and 2,5-dihydroxyacetophenone (DHAP) matrix." Rapid communications in mass spectrometry : RCM 33, 8 (2019): 811-819. Details.
  • Belgodere, JA ;Zamin, SA ;Kalinoski, RM ;Astete, CE ;Penrod, JC ;Hamel, KM ;Lynn, BC ;Rudra, JS ;Shi, J.;Jung, JP "Modulating Mechanical Properties of Collagen-Lignin Composites." ACS applied bio materials 2, 8 (2019): 3562-3572. Details.
  • Asare, SO ;Huang, F ;Lynn, BC "Characterization and sequencing of lithium cationized β-O-4 lignin oligomers using higher-energy collisional dissociation mass spectrometry." Analytica chimica acta 1047, (2019): 104-114. Details. Full text
  • Lovell, MA ;Lynn, BC ;Fister, S.;Bradley-Whitman, M ;Murphy, MP ;Beckett, TL ;Norris, CM "A Novel Small Molecule Modulator of Amyloid Pathology." Journal of Alzheimer's disease : JAD 53, 1 (2016): 273-87. Details. Full text
  • Kato, DM ;Elía, N ;Flythe, M ;Lynn, BC "Pretreatment of lignocellulosic biomass using Fenton chemistry." Bioresource technology 162, (2014): 273-8. Details. Full text
  • Dhar, SK ;Zhang, J.;Gal, J ;Xu, Y.;Miao, L ;Lynn, BC ;Zhu, H.;Kasarskis, EJ ;St Clair, DK "FUsed in sarcoma is a novel regulator of manganese superoxide dismutase gene transcription." Antioxidants & redox signaling 20, 10 (2014): 1550-66. Details. Full text
  • Li, HF ;Knutson, BL ;Nokes, SE ;Lynn, BC ;Flythe, MD "Metabolic control of Clostridium thermocellum via inhibition of hydrogenase activity and the glucose transport rate." Applied microbiology and biotechnology 93, 4 (2012): 1777-84. Details. Full text
  • Timmons, MD ;Bradley, MA ;Lovell, MA ;Lynn, BC "Procedure for the isolation of mitochondria, cytosolic and nuclear material from a single piece of neurological tissue for high-throughput mass spectral analysis." Journal of neuroscience methods 197, 2 (2011): 279-82. Details. Full text
  • Fu, YJ ;Xiong, S ;Lovell, MA ;Lynn, BC "Quantitative proteomic analysis of mitochondria in aging PS-1 transgenic mice." Cellular and molecular neurobiology 29, 5 (2009): 649-64. Details. Full text
  • Timmons, MD ;Knutson, BL ;Nokes, SE ;Strobel, HJ ;Lynn, BC "Analysis of composition and structure of Clostridium thermocellum membranes from wild-type and ethanol-adapted strains." Applied microbiology and biotechnology 82, 5 (2009): 929-39. Details. Full text
  • Liu, T.;Martin, AM ;Sinai, AP ;Lynn, BC "Three-layer sandwich gel electrophoresis: a method of salt removal and protein concentration in proteome analysis." Journal of proteome research 7, 10 (2008): 4256-65. Details. Full text
  • Nelson, WD ;Viele, K.;Lynn, BC "An integrated approach for automating validation of extracted ion chromatographic peaks." Bioinformatics (Oxford, England) 24, 18 (2008): 2103-4. Details. Full text
  • Sun, J.;Lynn, BC "Development of a MALDI-TOF-MS method to identify and quantify butyrylcholinesterase inhibition resulting from exposure to organophosphate and carbamate pesticides." Journal of the American Society for Mass Spectrometry 18, 4 (2007): 698-706. Details.
  • Williams, TI ;Combs, JC ;Lynn, BC ;Strobel, HJ "Proteomic profile changes in membranes of ethanol-tolerant Clostridium thermocellum." Applied microbiology and biotechnology 74, 2 (2007): 422-32. Details. Full text
  • Martin, AM ;Liu, T.;Lynn, BC ;Sinai, AP "Elimination of affinity reagent interference for the mass spectrometric detection of low-abundance proteins following immunoprecipitation." Journal of proteome research 6, 12 (2007): 4758-62. Details. Full text
  • Williams, TI ;Combs, JC ;Thakur, AP ;Strobel, HJ ;Lynn, BC "A novel Bicine running buffer system for doubled sodium dodecyl sulfate - polyacrylamide gel electrophoresis of membrane proteins." Electrophoresis 27, 14 (2006): 2984-95. Details.
  • Liu, X.;Lovell, MA ;Lynn, BC "Detection and quantification of endogenous cyclic DNA adducts derived from trans-4-hydroxy-2-nonenal in human brain tissue by isotope dilution capillary liquid chromatography nanoelectrospray tandem mass spectrometry." Chemical research in toxicology 19, 5 (2006): 710-8. Details. Full text
  • Williams, TI ;Lynn, BC ;Markesbery, WR ;Lovell, MA "Increased levels of 4-hydroxynonenal and acrolein, neurotoxic markers of lipid peroxidation, in the brain in Mild Cognitive Impairment and early Alzheimer's disease." Neurobiology of aging 27, 8 (2006): 1094-9. Details. Full text
  • Lovell, MA ;Geiger, H ;Van Zant, GE ;Lynn, BC ;Markesbery, WR "Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain." Neurobiology of aging 27, 7 (2006): 909-17. Details. Full text
  • Boyd-Kimball, D ;Poon, HF ;Lynn, BC ;Cai, J.;WM, Pierce ;Klein, JB ;Ferguson, J ;Link, CD ;Butterfield, DA "Proteomic identification of proteins specifically oxidized in Caenorhabditis elegans expressing human Abeta(1-42): implications for Alzheimer's disease." Neurobiology of aging 27, 9 (2006): 1239-49. Details. Full text
  • Pocernich, CB ;Poon, HF ;Boyd-Kimball, D ;Lynn, BC ;Nath, A.;Klein, JB ;Butterfield, DA "Proteomic analysis of oxidatively modified proteins induced by the mitochondrial toxin 3-nitropropionic acid in human astrocytes expressing the HIV protein tat." Brain research. Molecular brain research 133, 2 (2005): 299-306. Details. Full text
  • Liu, X.;Lovell, MA ;Lynn, BC "Development of a method for quantification of acrolein-deoxyguanosine adducts in DNA using isotope dilution-capillary LC/MS/MS and its application to human brain tissue." Analytical chemistry 77, 18 (2005): 5982-9. Details. Full text
  • Lovell, MA ;Xiong, S ;Markesbery, WR ;Lynn, BC "Quantitative proteomic analysis of mitochondria from primary neuron cultures treated with amyloid beta peptide." Neurochemical research 30, 1 (2005): 113-22. Details. Full text
  • Poon, HF ;Hensley, K ;Thongboonkerd, V ;Merchant, ML ;Lynn, BC ;Pierce, WM ;Klein, JB ;Calabrese, V.;Butterfield, DA "Redox proteomics analysis of oxidatively modified proteins in G93A-SOD1 transgenic mice--a model of familial amyotrophic lateral sclerosis." Free radical biology & medicine 39, 4 (2005): 453-62. Details. Full text
  • Pocernich, CB ;Boyd-Kimball, D ;Poon, HF ;Thongboonkerd, V ;Lynn, BC ;Klein, JB ;Calebrese, V ;Nath, A.;Butterfield, DA "Proteomics analysis of human astrocytes expressing the HIV protein Tat." Brain research. Molecular brain research 133, 2 (2005): 307-16. Details. Full text
  • Wenner, BR ;Lynn, BC "Factors that affect ion trap data-dependent MS/MS in proteomics." Journal of the American Society for Mass Spectrometry 15, 2 (2004): 150-7. Details.
  • Castegna, A ;Thongboonkerd, V ;Klein, J.;Lynn, BC ;Wang, YL ;Osaka, H ;Wada, K.;Butterfield, DA "Proteomic analysis of brain proteins in the gracile axonal dystrophy (gad) mouse, a syndrome that emanates from dysfunctional ubiquitin carboxyl-terminal hydrolase L-1, reveals oxidation of key proteins." Journal of neurochemistry 88, 6 (2004): 1540-6. Details. Full text
  • Liu, X.;Zhang, J.;Song, L ;Lynn, BC ;Burke, TG "Degradation of camptothecin-20(S)-glycinate ester prodrug under physiological conditions." Journal of pharmaceutical and biomedical analysis 35, 5 (2004): 1113-25. Details. Full text
  • Dhar, SK ;Lynn, BC ;Daosukho, C ;St Clair, DK "Identification of nucleophosmin as an NF-kappaB co-activator for the induction of the human SOD2 gene." The Journal of biological chemistry 279, 27 (2004): 28209-19. Details. Full text
  • Ambati, J ;Anand, A ;Fernandez, S ;Sakurai, E ;Lynn, BC ;Kuziel, WA ;Rollins, BJ ;Ambati, BK "An animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice." Nature medicine 9, 11 (2003): 1390-7. Details. Full text
  • Liu, X.;Lynn, BC ;Zhang, J.;Song, L ;Bom, D ;Du, W ;Curran, DP ;Burke, TG "A versatile prodrug approach for liposomal core-loading of water-insoluble camptothecin anticancer drugs." Journal of the American Chemical Society 124, 26 (2002): 7650-1. Details. Full text
  • Martin, AM ;Liu, T.;Lynn, BC ;Sinai, AP "The Toxoplasma gondii parasitophorous vacuole membrane: transactions across the border." The Journal of eukaryotic microbiology 54, 1 (1969): 25-8. Details. Full text
  • Poon, HF ;Castegna, A ;Farr, SA ;Thongboonkerd, V ;Lynn, BC ;Banks, WA ;Morley, JE ;Klein, JB ;Butterfield, DA "Quantitative proteomics analysis of specific protein expression and oxidative modification in aged senescence-accelerated-prone 8 mice brain." Neuroscience 126, 4 (0): 915-26. Details. Full text
  • Lynn, BC ;Wang, J.;Markesbery, WR ;Lovell, MA "Quantitative changes in the mitochondrial proteome from subjects with mild cognitive impairment, early stage, and late stage Alzheimer's disease." Journal of Alzheimer's disease : JAD 19, 1 (0): 325-39. Details. Full text
  • Bradley-Whitman, MA ;Roberts, KN ;Abner, EL ;Scheff, SW ;Lynn, BC ;Lovell, MA "A novel method for the rapid detection of post-translationally modified visinin-like protein 1 in rat models of brain injury." Brain injury 32, 3 (0): 363-380. Details. Full text
  • Boyd-Kimball, D ;Sultana, R.;Poon, HF ;Lynn, BC ;Casamenti, F ;Pepeu, G ;Klein, JB ;Butterfield, DA "Proteomic identification of proteins specifically oxidized by intracerebral injection of amyloid beta-peptide (1-42) into rat brain: implications for Alzheimer's disease." Neuroscience 132, 2 (0): 313-24. Details. Full text
* Publications are automatically pulled from pubmed.gov based on a user-specific query. Results may include incorrect citations. See: Tutorial on improving PubMed results.