Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues.
| Author | |
|---|---|
| Abstract | :
Hypoxia (insufficient O2 availability) is involved in various biological processes, such as tumorigenesis and inflammation. Hypoxia results in stabilization of hypoxia-inducible factors (HIFs) including HIF1α and HIF2α. Here we describe a protocol to detect mouse and human tissue hypoxia by using Hypoxyprobe and immunohistochemical staining for HIF1α and HIF2α. |
| Year of Publication | :
0
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| Journal | :
Methods in molecular biology (Clifton, N.J.)
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| Volume | :
1742
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| Number of Pages | :
277-282
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| Date Published | :
2018
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| ISSN Number | :
1064-3745
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| URL | :
https://dx.doi.org/10.1007/978-1-4939-7665-2_24
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| DOI | :
10.1007/978-1-4939-7665-2_24
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| Short Title | :
Methods Mol Biol
|
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