Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA.
| Author | |
|---|---|
| Abstract | :
An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids. |
| Year of Publication | :
2018
|
| Journal | :
Journal of microbiological methods
|
| Date Published | :
2018
|
| ISSN Number | :
0167-7012
|
| URL | :
http://linkinghub.elsevier.com/retrieve/pii/S0167-7012(18)30009-5
|
| DOI | :
10.1016/j.mimet.2018.01.005
|
| Short Title | :
J Microbiol Methods
|
| Download citation |