Jason DeRouchey is an Assistant Professor in the Department of Chemistry at the University of Kentucky. He received his B.S. degree in Chemistry from the University of Texas at Dallas in 1996. Professor DeRouchey then obtained a MS and PhD in Polymer Science and Engineering at the University of Massachusetts-AmHerst. He first began working with questions of dynamics and DNA as a Alexander von Humboldt Fellow working with Joachim Rädler at the Institute of Experimental Physics at the Ludwig Maximilians Universität (LMU) Munich. Dr. DeRouchey then joined the Laboratory of Physical and Structural Biology at the National Institute of Child Health & Human Development (NICHD) at the National Institutes of Health (NIH) as a IRTA fellow working with V. Adrian Parsegian.
The DeRouchey laboratory is broadly interested in understanding the forces, structures, and dynamics that govern the interaction of biologically significant macromolecular assemblies to address problems in biology and biomedicine. Using interdisciplinary methodologies in chemistry, physics, and medicine, we focus currently on issues related to DNA condensation in vitro and in vivo and the delivery of artificial gene constructs. Learning the strength, specificity, and reversibility in associates of biologically important macromolecules, typically in crowded environments, is crucial to our understanding of gene and cellular function and for effective and rational drug design.
For more information, please visit the DeRouchey Group website.
DeRouchey, J.; Hoover, B.; Rau, D. A comparison of DNA compaction by arginine and lysine peptides: A physical basis for arginine rich protamines. Biochemistry. 2013, 52 (17), 3000-3009.
DeRouchey, J.; Rau, D. Role of amino acid insertions on intermolecular forces between arginine peptide condensed DNA helices: Implications for protamine-DNA packaging in sperm. J Biol. Chem. 2011, 286 (49) 41985-41992.
DeRouchey, J.; Rau, D. Salt Effects on Condensed Protamine-DNA Assemblies: Anion Binding and Weakening of Attraction. J Phys Chem B. 2011, 115(41):11888-94.
- DeRouchey, J.; Parsegian, V. A.; Rau, D. Cation Charge Dependence of the Forces Driving DNA Assembly. Biophysical Journal, 2010, in press.Hoenig, D.;
- DeRouchey, J.; Jungmann, R.; et al. Biophysical Characterization of Copolymer Protected Gene Vectors (COPROGs). Biomacromolecules. 2010, 11(7), 1802-1809.
- Podgornik, R.; Harries, D.; DeRouchey, J.; Parsegian, V. A.; Strey, H. H. Interactions in Macromolecular Complex Used as Nonviral Vectors for Gene Delivery. In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies; Third Edition. Templeton, N.S. ed; Marcel Dekker: New York, 2009; pp 443-484.
- DeRouchey, J.; Schmidt, C.; Walker, G. F.; Plank, C.; Wagner, E.; Rädler, J. O. Monomolecular Assembly of siRNA and Poly(ethyleneglycol)-Peptide Copolymers. Biomacromolecules. 2008, 9 (2), 724-732.
- Pelisek, J.; Gaedtke, L.; DeRouchey, J.; et al. Optimized Lipopolyplex Formulations for Gene Transfer To Human Colon Carcinoma Cells Under in vitro Conditions. J. Gene Med. 2006, 8 (2), 186-197.
- DeRouchey, J.; Walker, G. F.; Wagner, E.; Rädler J. O.; Decorated Rods: A "Bottom-Up" Self- Assembly of Monomolecular DNA Complexes. J. Phys. Chem. B. 2006, 110 (10), 4548-4554.
- DeRouchey, J.; Netz, R. R.; Rädler J. O.; Structural Investigations of DNA-Polycation Complexes. European Physics Journal E – Soft Matter and Biological Physics. 2005, 16 (1), 17-28.
- DeRouchey, J.; Thurn-Albrecht, T.; Russell, T. P.; Kolb, R., "Block Copolymer Domain Reorientation In An Electric Field: An In-situ Small-Angle X-ray Scattering Study.“ Macromolecules. 2004, 37 (7), 2538-2543.
- Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; Kolb, R. Pathways towards electric field induced alignment of block copolymers. Macromolecules. 2002, 35 (21), 8106-8110.
- Thurn-Albrecht, T.; Steiner, R.; DeRouchey, J.; et al. Nanoscopic Templates from Oriented Block Copolymer Films. Advanced Materials. 2000, 12 (11), 787-791.
- Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; et al. Overcoming Interfacial Interactions with Electric Fields. Macromolecules. 2000, 33(9), 3250-3253.
- Boal, A.K.; Ilhan, F.; DeRouchey, J. E.; et al. Self-assembly of Nanoparticles into Structured Spherical and Network Aggregates. Nature. 2000, 404 (6779), 746-748.